研究業種(論文発表)

Zhang B, Azuma J, Takeno S, Suzuki N, Nakazawa Y, Uyama H (2016) Improvement of the rheological properties of trans-1,4-polyisoprene from Eucommia ulmoides Oliver by tri-branched poly(ricinoleic acid). Polymer J (in press)

Takeno KJ, Takeno S, Yuki I, Yamaguchi S, Suzuki N, Nakazawa Y (2016) Rapid determination of anhydride content in trans-polyisoprene-graft-maleic anhydride by double-shot pyrolysis gas chromatography–mass spectrometry. J Anal Appl Pyrolysis 117:363–370

Tokumoto Y, Kajiura H, Takeno S, Harada Y, Suzuki N, Hosaka T, Gyokusen K, Nakazawa Y (2016) Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid. Plant Biotechnol 33:51-57

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Tsujimoto T, Toshimitsu K, Uyama H, Takeno S, Nakazawa Y (2014) Maleated trans-1,4-polyisoprene from Eucommia ulmoides Oliver with dynamic network structure and its shape memory property. Polymer 55:6488-6493

Yamamoto N, Kajiura H, Takeno S, Suzuki N, Nakazawa Y (2014) A watermarking system for labeling genomic DNA. Plant Biotechnol 31:241-248

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Nakazawa Y, Takeda T, Suzuki N, Hayashi T, Harada Y, Bamba T, Kobayashi A (2013) Histochemical study of trans-polyisoprene accumulation by spectral confocal laser scanning microscopy and a specific dye showing fluorescence solvatochromism in the rubber-producing plant, Eucommia ulmoides Oliver. Planta 238:549-560

Kitade Y, Okino S, Gunji W, Hiraga K, Suda M, Suzuki N, Inui M, Yukawa H (2013) Identification of a gene involved in plasmid structural instability in Corynebacterium glutamicum. Appl Microbiol Biotechnol 97:8219-8226

Okibe N, Suzuki N, Inui M, Yukawa H (2013) pCGR2 copy number depends on the par locus that forms a ParC-ParB-DNA partition complex in Corynebacterium glutamicum. J Appl Microbiol 115:495-508

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, and Takaiwa F (2013) Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies. Plant Cell Physiol 54:917-933

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N (2013) Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks. PLoS Negl Trop Dis 7:e2046

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K (2013) N-Glycosylation and N-Glycan Moieties of CTB Expressed in Rice Seeds. Mol Biotechnol 54:784-794

Watanabe K, Teramoto H, Suzuki N, Inui M, Yukawa H (2013) Influence of SigB inactivation on Corynebacterium glutamicum protein secretion. Appl Microbiol Biotechnol 97: 4917-4926

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Chen R, Harada Y, Bamba T, Nakazawa Y, Gyokusen K (2012) Overexpression of an isopentenyl diphosphate isomerase gene to enhance trans-polyisoprene production in Eucommia ulmoides Oliver. BMC Biotechnol 30: 12-78

Suzuki N, Uefuji H, Nishikawa T, Mukai Y, Yamashita A, Hattori M, Ogasawara N, Bamba T, Fukusaki E, Kobayashi A, Ogata Y, Sakurai N, Suzuki H, Shibata D, Nakazawa Y (2012) Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver. Planta 236:1405-1417

Uematsu K, Suzuki N, Iwamae T, Inui M, Yukawa H (2012) Expression of Arabidopsis plastidial phosphoglucomutase in tobacco stimulates photosynthetic carbon flow into starch synthesis. J Plant Physiol 169:1454-1462

Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T and Fujiyama K (2012) N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells. Plant Biotechnol 29: 489-493

Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K and Li J (2012) Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis. Proc Natl Acad Sci USA 109:11437-11442

Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma JK, Benvenuto E (2012) Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation. Transgenic Res 21:1005-1021

Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H and Omasa T (2012) Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of a2,6-sialyltransferase derived from Chinese hamster ovary cells. Appl Microbiol Biotechnol 94:69-80

Uematsu K, Suzuki N, Iwamae T, Inui M, Yukawa H (2012) Alteration of photosynthate partitioning by high-level expression of phosphoglucomutase in tobacco chloroplasts. Biosci Biotechnol Biochem 76:1315-1321

Kajiura H, Okamoto T, Misaki R, Matsuura Y and Fujiyama K (2012) Arabidopsis b1,2-Xylosyltransferase: substrate specificity and participation in the plant-specific N-glycosylation pathway. J Biosci Bioeng 113:48-54

Uematsu K, Suzuki N, Iwamae T, Inui M, Yukawa H (2012) Increased fructose 1,6-bisphosphate aldolase in plastids enhances growth and photosynthesis of tobacco plants. J Exp Bot 63:3001-3009

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Teramoto H, Watanabe K, Suzuki N, Inui M, Yukawa H (2011) High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence. Appl Microbiol Biotechnol 91:677-687

Kaulfuerst-Soboll H, Rips S, Koiwa H, Kajiura H, Fujiyama K, von Schaewen A (2011) Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes. J Biol Chem 286:22955-22964

Kajiura H, Misaki R, Fujiyama K and Seki T (2011) Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells. J Biosci Bioeng 111:471-477

Okibe N, Suzuki N, Inui M, Yukawa H (2011) Efficient markerless gene replacement in Corynebacterium glutamicum using a new temperature-sensitive plasmid. J Microbiol Methods 85:155-163

Niimi S, Suzuki N, Inui M, Yukawa H (2011) Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. Appl Microbiol Biotechnol 90:1721-1729

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Takeno S, Bamba T, Nakazawa Y, Fukusaki E, Okazawa A, Kobayashi A (2010) High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry. Biosci Biotechnol Biochem 74:13-17

Kajiura H, Koiwa H, Nakazawa Y, Okazawa A, Kobayashi A, Seki T, Fujiyama K (2010) Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation. Glycobiology 20:235-247

Kajiura H, Seki T and Fujiyama K (2010) Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans. Glycobiology 20:736-751

Mitsui T, Asakura T, Nakayama Y, Takayama K, Hirose S, Katamine H, Aoyama M, Karahashi A, Kaneko K, Ishiyama R, Itoh K, Hori H, Kajiura H and Fujiyama K (2010) Rice Golgi Proteome: Identification and Characterization of N-acetylglucosaminyltransferase-I-like Protein. Bull. Facul. Agric. Niigata Univ. 63:19-27

Khalil MF, Kajiura H, Fujiyama K, Koike K, Ishida N and Tanaka N (2010) The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants. J Biosci Bioeng 109:159-169

Chen R, Gyokusen M, Nakazawa Y, Su Y, Gyokusen K (2010) Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge. Plant Biotechnology 27:173-181

Chen R, Gyokusen M, Nakazawa Y, Gyokusen K (2010) Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR. Journal of Botany 2010:7

Bamba T, Murayoshi M, Gyoksen K, Nakazawa Y, Okumoto H, Katto H, Fukusaki E, Kobayashi A (2010) Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver. Z Naturforsch C 65: 363-372

Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H (2010) Characterization of a 24-kb plasmid pCGR2 newly isolated from Corynebacterium glutamicum. Appl Microbiol Biotechnol 87:1855-1866

Okibe N, Suzuki N, Inui M, Yukawa H (2010) Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum. Lett Appl Microbiol 50:173-180

Okibe N, Suzuki N, Inui M, Yukawa H (2010) Antisense-RNA-mediated plasmid copy number control in pCG1-family plasmids, pCGR2 and pCG1, in Corynebacterium glutamicum. Microbiology 156:3609-3623

Kim WD, Tokunaga M, Ozaki H, Ishibashi T, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Omasa T and Ohtake H (2010) Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells. Appl Microbiol Biotechnol 85:535-542

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Sando T, Hayashi T, Takeda T, Akiyama Y, Nakazawa Y, Fukusaki E, Kobayashi A (2009) Histochemical study of detailed laticifer structure and rubber biosynthesis-related protein localization in Hevea brasiliensis using spectral confocal laser scanning microscopy. Planta 230:215-225

Misaki R, Kajiura H, Fujii K, Fujiyama K and Seki T (2009) Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana. J Biosci Bioeng 108:527-529

Matoba N, Kajiura H, Cherni I, Doran JD, Bomsel M, Fujiyama K and Mor TS (2009) Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR. Plant Biotechnol J 7:129-145>

Nakazawa Y, Bamba T, Takeda T, Uefuji H, Harada Y, Li X, Chen R, Inoue S, Tutumi M, Shimizu T, Su Y, Gyokusen K, Fukusaki E, Kobayashi A (2009) Production of Eucommia-rubber from Eucommia ulmoides Oliv. (Hardy Rubber Tree). Plant Biotechnol 26:71-79

Watanabe K, Tsuchida Y, Okibe N, Teramoto H, Suzuki N, Inui M, Yukawa H (2009) Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences. Microbiology 155:741-750

Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H (2009) Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector. Appl Microbiol Biotechnol 81:1107-1115

Suzuki N, Watanabe K, Okibe N, Tsuchida Y, Inui M, Yukawa H (2009) Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum. Appl Microbiol Biotechnol 82:491-500

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Takeno S, Bamba T, Nakazawa Y, Fukusaki E, Okazawa A, Kobayashi A (2008) Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry. J Biosci Bioeng 105:355-359

Kang JS, Frank J, Kang CH, Kajiura H, Vikram M, Ueda A, Kim S, Bahk JD, Triplett B, Fujiyama K, Lee SY, von Schaewen A and Koiwa H (2008) Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus. Proc Natl Acad Sci USA 105:5933-5938

Yang L, Kajiura H, Suzuki K, Hirose S, Fujiyama K and Takaiwa F (2008) Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Biochem Biophys Res Commun 365:334-339

Takeno S, Bamba T, Nakazawa Y, Fukusaki E, Okazawa A, Kobayashi A (2008) A high-throughput and solvent-free method for measurement of natural polyisoprene content in leaves by Fourier transform near infrared spectroscopy. J Biosci Bioeng 106:537-540

Chen R, Namimatsu S, Nakadozono Y, Bamba T, Nakazawa Y, Gyokusen K (2008) Efficient regeneration of Eucommia ulmoides from hypocotyl explant. BIOLOGIA PLANTARUM 52:713-717

Bamba T, Shimonishi N, Matsubara A, Hirata K, Nakazawa Y, Kobayashi A, Fukusaki E (2008) High throughput and exhaustive analysis of diverse lipids by using supercritical fluid chromatography-mass spectrometry for metabolomics. J Biosci Bioeng 105:460-469

Sando T, Takeno S, Watanabe N, Okumoto H, Kuzuyama T, Yamashita A, Hattori M, Ogasawara N, Fukusaki E, Kobayashi A (2008) Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis. Biosci Biotechnol Biochem 72:2903-2917

Hasunuma T, Takeno S, Hayashi S, Sendai M, Bamba T, Yoshimura S, Tomizawa K, Fukusaki E, Miyake C (2008) Overexpression of 1-Deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production. J Biosci Bioeng 105:518-526

Suzuki N, Inui M, Yukawa H (2008) Random genome deletion methods applicable to prokaryotes. Appl Microbiol Biotechnol 79:519-526

Inui M, Suda M, Kimura S, Yasuda K, Suzuki H, Toda H, Yamamoto S, Okino S, Suzuki N, Yukawa H (2008) Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl Microbiol Biotechnol 77:1305-1316

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Jin-Nian P, Yin-Quan S, Qi-Shi S, YUE J, Nakazawa Y, Run-Cang S (2007) Content and molecular-weight distribution of EU rubber from Eucommia ulmoides leaves. Forest products journal 57:65-67

Kimura Y, Sakamura S, Ushijima T, Hama Y, Kajiura H, Fujiyama K, Okihara K, Hashimoto K, Sugimoto H and Yamada H (2007) Evidence for new b1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera. Biosci Biotechnol Biochem 71: 1111-1114

Bamba T, Sando T, Miyabashira A, Gyokusen K, Nakazawa Y, Su Y, Fukusaki E, Kobayashi A (2007) Periploca sepium Bunge as a model plant for rubber biosynthesis study. Z Naturforsch C 62:579-582

Yukawa H, Omumasaba CA, Nonaka H, Kos P, Okai N, Suzuki N, Suda M, Tsuge Y, Watanabe J, Ikeda Y, Vertes AA, Inui M (2007) Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R. Microbiology 153:1042-1058

Tsuge Y, Suzuki N, Inui M, Yukawa H (2007) Random segment deletion based on IS31831 and Cre/loxP excision system in Corynebacterium glutamicum. Appl Microbiol Biotechnol 74:1333-1341

Tsuge Y, Suzuki N, Ninomiya K, Inui M, Yukawa H (2007) Isolation of a new insertion sequence, IS13655, and its application to Corynebacterium glutamicum genome mutagenesis. Biosci Biotechnol Biochem 71:1683-1690

Suzuki N, Inui M, Yukawa H (2007) Site-directed integration system using a combination of mutant lox sites for Corynebacterium glutamicum. Appl Microbiol Biotechnol 77:871-878

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Suzuki N, Okai N, Nonaka H, Tsuge Y, Inui M, Yukawa H (2006) High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library. Appl Environ Microbiol 72:3750-3755

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Hirooka K, Izumi Y, An CI, Nakazawa Y, Fukusaki E, Kobayashi A (2005) Functional analysis of two solanesyl diphosphate synthases from Arabidopsis thaliana. Biosci Biotechnol Biochem 69:592-601

Bamba T, Fukusaki E, Minakuchi H, Nakazawa Y, Kobayashi A (2005) Separation of polyprenol and dolichol by monolithic silica capillary column chromatography. J Lipid Res 46:2295-2298

Tsuge Y, Ninomiya K, Suzuki N, Inui M, Yukawa H (2005) A new insertion sequence, IS14999, from Corynebacterium glutamicum. Microbiology 151:501-508

Suzuki N (2005) Alleviation by calcium of cadmium-induced root growth inhibition in Arabidopsis seedlings. Plant Biotechnol 22:19-25

Suzuki N, Tsuge Y, Inui M, Yukawa H (2005) Cre/loxP-mediated deletion system for large genome rearrangements in Corynebacterium glutamicum. Appl Microbiol Biotechnol 67:225-233

Suzuki N, Nonaka H, Tsuge Y, Okayama S, Inui M, Yukawa H (2005) Multiple large segment deletion method for Corynebacterium glutamicum. Appl Microbiol Biotechnol 69:151-161

Suzuki N, Okayama S, Nonaka H, Tsuge Y, Inui M, Yukawa H (2005) Large-scale engineering of the Corynebacterium glutamicum genome. Appl Environ Microbiol 71:3369-3372

Suzuki N, Nonaka H, Tsuge Y, Inui M, Yukawa H (2005) New multiple-deletion method for the Corynebacterium glutamicum genome, using a mutant lox sequence. Appl Environ Microbiol 71:8472-8480

Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H (2005) Isolation and characterization of a native composite transposon, Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum chromosomal DNA. Appl Environ Microbiol 71:407-416

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Bamba T, Fukusaki E, Nakazawa Y, Kobayashi A (2004) Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography. J Sep Sci 27:293-296

Fukusaki E, Takeno S, Bamba T, Okumoto H, Katto H, Kajiyama S, Kobayashi A (2004) Biosynthetic pathway for the C45 polyprenol, solanesol, in tobacco. Biosci Biotechnol Biochem 68:1988-1990

Garbe TR, Suzuki N, Inui M, Yukawa H (2004) Inhibitor-associated transposition events in Corynebacterium glutamicum. Mol Genet Genomics 271:729-741

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Bamba T, Fukusaki E, Nakazawa Y, Sato H, Ute K, Kitayama T, Kobayashi A (2003) Analysis of long-chain polyprenols using supercritical fluid chromatography and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Chromatogr A 995:203-207

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Bamba T, Fukusaki E, Nakazawa Y, Kobayashi A (2002) In-situ chemical analyses of trans-polyisoprene by histochemical staining and Fourier transform infrared microspectroscopy in a rubber-producing plant, Eucommia ulmoides Oliver. Planta 215:934-939

Suzuki N, Yamaguchi Y, Koizumi N, Sano H (2002) Functional characterization of a heavy metal binding protein CdI19 from Arabidopsis. Plant J 32:165-173

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Deyama T, Nishibe S, Nakazawa Y (2001) Constituents and pharmacological effects of Eucommia and Siberian ginseng. Acta Pharmacol Sin 22:1057-1070

Suzuki N, Koizumi N, Sano H (2001) Screening of cadmium-responsive genes in Arabidopsis thaliana. Plant Cell Environ 24:1177-1188

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Tanaka C, Nakamura T, Nakazawa Y, Nohara T (1997) A New Triterpenoid from the Leaves of Eucommia ulmoides OLIV. Chemical and pharmaceutical bulletin 45:1379-1380

Nakamura T, Nakazawa Y, Onizuka S, Satoh S, Chiba A, Sekihashi K, Miura A, Yasugahira N, Sasaki YF (1997) Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 1. The clastogen-suppressing effects of Tochu tea in CHO cells and mice. Mutat Res 388:7-20

Sasaki YF, Chiba A, Murakami M, Sekihashi K, Tanaka M, Takahoko M, Moribayashi S, Kudou C, Hara Y, Nakazawa Y, Nakamura T, Onizuka S (1996) Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 2. Suppressing effect of Tochu tea on the urine mutagenicity after ingestion of raw fish and cooked beef. Mutat Res 371:203-214

Yahara S, Kato K, Nakazawa Y, Toda Y, Nohara T (1990) New iridoid trimers and tetramers from seeds of Eucommia ulmoides. Chemical and Pharmaceutical Bulletin 38:267-269

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